第一届广州核酸国际论坛

2013年9月11-13日(已结束)      广州 天河希尔顿酒店

议题介绍

NGS技术是核酸测序领域的一个新突破。它采用成本相对低廉的高通量测序,取代了以往耗时费力,成本昂贵的低通量方法。这项技术催生了当前测序方法的概念范式转变,同时也促进了两项新的关键应用的快速发展——全基因组测序和目标区域测序。在采用NGS技术之前,要破译基因组序列,即便目标微小如细菌,也需要动用多个研究小组协作,并要耗费数个月,甚至数年的时间才能完成。而如今,即便是真核生物这样的大型基因组也能在几周内由单一研究小组完成测序。目标区域测序使得研究人员能够精确获取特定区域或是特定核酸种类的详细数据,这对于非编码RNA的研究尤其有益。在短时间内提供大量测序信息需要更先进的途径和方法,对生物信息学专家提出了新的挑战。本届论坛将揭示NGS和生物信息学对新型测序数据分析的最新趋势,并重点关注非编码RNA。

论坛议程
8:50 – 9:00 开幕式——丹米其.萨玛斯基(Dmitry Samarsky)博士和张必良(Biliang Zhang)博士(CNAF 组织者)
第一天报告: 主持人:付向东(Xiang-dong Fu)博士和李蔚(Wei Li)博士
9:00 – 9:40 主旨报告 克雷格.梅洛(Craig C. Mello)博士

2006诺贝尔生理学或医学奖获得者,美国麻省大学医学院教授

题目:CSR-1 mediates RNA activation, RNAa, a self-RNA recognition pathway that promotes gene expression in the C. elegans germline

9:40 – 10:20 刘默芳(Mo-fang Liu)博士

中国科学院上海生命科学研究院生物化学与细胞生物学研究所研究员

题目:Spermiogenesis requires piRNA-triggered MIWI ubiquitination and removal by APC/C

10:20 – 10:40 茶歇
10:40 – 11:20 杨力(Li Yang)博士

中国科学院上海生命科学研究院计算生物学研究所研究员

题目:Pan for non-polyadenylated long noncoding RNAs

11:20 – 12:00 付向东(Xiang-dong Fu)博士

美国加州大学圣地亚哥分校教授

题目:A surprising role of nascent RNA(待定)

12:00 – 14:00 午餐
14:00 – 14:40 李蔚(Wei Li)博士

美国贝勒医学院副教授

题目:Dynamic analysis of Alternative PolyAdenylation from RNA-Seq (DaPars) Reveals Global Shortening of 3’UTRs in Human Cancers

14:40 – 15:20 布莱恩.哈斯(Brian J. Haas)博士

博德研究所高级计算生物学家

题目:A hybrid approach to comprehensive transcriptome reconstruction using genome-guided and de novo RNA-Seq assembly leveraging Trinity and PASA2

15:20 -15:40 茶歇
15:40 -16:20 张弓(Gong Zhang)博士

暨南大学生命与健康工程研究院教授

题目:Genome-wide characterization of elongation velocity and translation ratio revealed polarized modes of translational control

16:20 -17:00 张晓华(Douglas Zhang)博士

默克研究院亚太区早期发育统计研究主管
题目:RNAi High-Throughput Screening and Quantitative Analysis

17:00 -17:10 第一天报告结束陈词——CNAF科学委员会
17:10 -19:10 晚餐

2013年9月13日,星期五

8:50 -9:00 第一天报告总结及第二天报告介绍——屈良鹄(Lianghu Qu)博士(CNAF 组织者)
第二天报告 主持人:翁志萍(Zhiping Weng)博士和迈克尔.麦克马纳斯(Michael McManus)博士
9:00 – 9:40 主旨报告 卡洛.克罗斯(Carlo M. Croce)博士

美国俄亥俄州立大学教授

题目:Causes and consequences of microRNA dysregulation in cancer

9:40 – 10:20 张戈(Ge Zhang)博士

香港浸会大学副教授
题目:Therapeutically targeting miR-214 in osteogenic cells promotes bone formation in mice with established osteoporosis induced by ovariectomy

10:20 – 10:40 茶歇
10:40 – 11:20 帕万.库马尔(Pavan Kumar)博士

卫材公司(美国)高级科学家

题目:Circulating miRNA biomarkers: An industrial perspective

11:20 – 12:00 朱景德(Jingde Zhu)博士

安徽肿瘤医院教授

题目:DNA methylation regulated miRs in cancer chemo-resistance:The miR-193a in bladder cancer paradigm

12:00 – 14:00 午餐
14:00 – 14:40 迈克尔.麦克马纳斯(Michael McManus)博士

美国加利福尼亚大学旧金山分校副教授

题目:Mammalian Interference Pathways

14:40 – 15:20 汪阳明(Yangming Wang)博士

北京大学教授

题目:MicroRNA Regulation of Cell Cycle and Self-Renewal in Embryonic Stem Cells

15:20 -15:40 茶歇
15:40 – 16:20 宋尔卫(Erwei Song)博士

中山大学教授

题目:Targeted Delivery of PLK1-siRNA by ScFv Suppresses Her2+ Breast Cancer Growth and Metastasis

16:20 – 17:00 翁志萍(Zhiping Weng)博士

美国麻省大学医学院教授

题目:PiRNAs and Genome Instability

17:00 -17:10 闭幕式——克雷格.梅洛(Craig C. Mello)博士(CNAF 科学委员会联执主席)
17:10 -19:10 晚餐
20:00 -21:30 珠江夜游(自选)

演讲嘉宾

卡洛•克罗斯(Carlo M. Croce)博士

美国俄亥俄州立大学(The Ohio State University)教授、遗传研究所所长

美国国家科学院院士,意大利国家科学院外籍院士

 

学术背景

Dr. Carlo M. Croce is the Professor and Chair of Molecular Virology, Immunology and Medical Genetics, Director of the Human Cancer Genetics Program, and Director of the Institute of Genetics at The Ohio State University Comprehensive Cancer Center. His research has revealed the variety of mutated genes involved in leukemias, lymphomas and other cancers.

Dr. Croce is world-renowned for his contributions involving the genes and genetic mechanisms implicated in the pathogenesis of human cancer. During the course of his career, he discovered the juxtaposition of the human immunoglobulin genes to the MYC oncogene, the deregulation of MYC in Burkitt lymphoma, the ALL1 gene involved in acute leukemias, the TCL1 gene associated with T-cell leukemias, and cloned and characterized the BCL2 gene involved in follicular lymphoma.

Dr. Croce has also uncovered the early events involved in the pathogenesis of lung, nasopharyngeal, head and neck, esophageal, gastrointestinal and breast cancers. His discoveries have led to revolutionary innovations in the development of novel and successful approaches to cancer prevention, diagnosis, monitoring and treatment, based on gene-target discovery, verification and rational drug development.

Dr. Croce is a member of the National Academy of Sciences, Institute of Medicine, American Academy of Arts and Sciences and an AAAS Fellow. He is principal investigator on seven federal research grants and has more than 1000 peer-reviewed, published research papers.

付向东(Xiang-dong Fu)博士

美国加州大学圣地亚哥分校(University of California, San Diego)教授

 

学术背景

Dr. Xiang-Dong Fu is Professor of Cellular and Molecular Medicine at University of California, San Diego. Dr. Fu received his MS degree in Virology from Wuhan University, China in 1982, PhD degree in Biochemistry from Case Western Reserve University in 1988 (via the CUSBEA program), and postdoctoral training at Harvard from 1988 to 1992. Dr. Fu joined the faculty of University of California, San Diego in 1992.

Dr. Fu was responsible for co-discovery of SR proteins. His laboratory was the first to identify a family of kinases specific for SR proteins and demonstrated that these kinases are critical for transducing external and intracellular signals to regulate alternative splicing in the nucleus. Dr. Fu’s group elucidated a series of regulatory mechanisms for splice site selection in mammalian cells and developed multiple key technologies for high throughput analysis of gene expression, mRNA isoforms, and genomic interactions. Dr. Fu’s current research is focused on integrated regulation of gene expression at transcriptional and post-transcriptional levels.

布莱恩•哈斯博士(Brian J. Haas)博士

博德研究所(Broad Institute)高级计算生物学家

 

学术背景

Dr. Brian Haas is a Senior Computational Biologist in the Klarman Cell Observatory at the Broad Institute, where he supports genome and transcriptome sequence analysis and related software development. Dr. Brian obtained a MS in molecular biology from SUNYA and a MS in computer science from Johns Hopkins University, and has pursued a career in genomics and bioinformatics since 1999. Dr. Brian is an active member of the bioinformatics open source software development community and is a lead developer of the Trinity RNA-Seq assembly (http://trinityrnaseq.sf.net) and PASA transcript-based genome annotation (http://pasa.sf.net) software projects.

帕万•库马尔(Pavan Kumar)博士

卫材公司(美国)(Eisai Research Institute)高级科学家

 

学术背景

Dr. Kumar earned his Ph.D. in plant molecular biology (with a designated emphasis in biotechnology) from the University of California at Davis in 2008. He then transitioned to molecular diagnostics and therapeutics when he joined Somagenics Inc, a startup company in Santa Cruz, CA. He helped develop a small RNA PCR detection platform, which was the first in class to be able to differentiate between 2’OMe modified small RNAs (piRNAs, a proportion of plant miRNAs for example) and standard 2′-OH small RNAs. This resulted in development of over 50 miRNA assays which are under evaluation and testing with both academic and industrial partners and a patent application. He then moved to Andover, MA in Nov 2010, where he joined Eisai Inc to lead efforts to develop miRNAs as potential biomarkers, and help integrate them with the company pipeline in addition to serving as potential stand alone diagnostics. This has recently led to both a publication and patent application for novel circulating miRNA biomarkers for identifying Alzheimer Disease patients. He also leads their next-generation sequencing efforts which are based on the Ion Torrent PGM platform.

李蔚(Wei Li)博士

美国贝勒医学院(Baylor College of Medicine)副教授

 

学术背景

Dr. Wei Li is an Associate Professor in the Dan L. Duncan Cancer Center and Department of Molecular and Cellular Biology at Baylor College of Medicine. Dr. Li received his PhD in Bioinformatics from the Chinese Academy of Sciences and postdoctoral training in the Department of Biostatistics and Computational Biology at the Dana-Farber Cancer Institute and Harvard School of Public Health. Dr. Li’s research is focused on the design and application of statistical and computational algorithms to elucidate epigenetic mechanisms in various disease models such as cancer. He has published more than 80 peer-reviewed papers, including 16 in Nature, Science, and Cell series, with a Google Scholar H-index of 31.

刘默芳(Mo-fang Liu)博士

中国科学院上海生命科学研究院生物化学与细胞生物学研究所研究员

 

学术背景

Dr. Mo-Fang Liu received her Ph.D degree in Biochemistry and Molecule Biology in 2000 from Shanghai Institute of Biochemistry, CAS. Dr. Liu was a postdoctoral fellow at National Cancer Institute, NIH, from 2000 to 2004. She joined Institute of Biochemistry and Cell Biology, SIBS, CAS, in 2006. The major research interests in her laboratory are the pathological function of miRNAs in cancer and the physiological roles of piRNAs in mammal spermatogenesis. In their recent works, they indicate that miR-155 acts as a bridge between inflammation and cancer through targeting socs1 and activating STAT3 through the JAK pathway (Cancer Res, 2010). Furthermore, they reveal that miR-155 links inflammation and cancer cell energy metabolism through up-regulation of hexokinase-2, a key glycolytic enzyme and pivotal player in the Warburg effect (EMBO J, 2012). Their most recent study shows that piRNAs trigger MIWI ubiquitination and removal by APC/C in late spermatogenesis and proper removal of MIWI/piRNA is essential for sperm maturation (Dev Cell, 2013).

迈克尔•麦克马纳斯(Michael McManus)博士

加利福尼亚大学(University of California, San Francisco)副教授

W.M. Keck中心创始人及主任

 

学术背景

Dr. McManus obtained his Ph.D. from the University of Alabama in Birmingham, where he studied RNA editing in the laboratory of Stephen L. Hajduk. He did his postdoctoral training as a Cancer Research Institute fellow, in the laboratory of Nobel Laureate Phillip A. Sharp at the Massachusetts Institute of Technology (MIT), studying the role of RNA-interference pathways in mammals. He is the Stella Coates Endowed Chair and appointed as an Associate Professor at the University of California San Francisco, in the Department of Microbiology and Immunology. He is the founder and Director of the W.M. Keck Center for Noncoding RNAs and also oversees the Lentiviral RNAi Core at UCSF. He has a long-standing interest in post-transcriptional gene regulation and the role of small RNAs in gene expression and carries almost 20 years experience working with noncoding RNA and the regulation of gene expression. Over the last five years to develop high-throughput screening approaches and developing/studying mouse gene knockout models for noncoding RNAs.

克雷格•梅洛(Craig C. Mello)博士

美国麻省大学医学院(University of Massachusetts Medical School)杰出教授、RNA治疗研究所主任

霍华德•休斯医学研究院(Howard Hughes Medical Institute)研究员

美国国家科学院院士、2006诺贝尔生理学或医学奖获得者

揭示了双链核糖核酸(dsRNA)基因沉默现象以及最近突破性发现piRNA诱导的表观遗传沉默途径。

 

学术背景

Dr. Craig C. Mello is an Investigator of the Howard Hughes Medical Institute, the Blais University Chair in Molecular Medicine and Co-director of the RNA Therapeutics Institute at the University of Massachusetts Medical School.

Dr. Mello’s lab uses the nematode C. elegans as a model system to study embryogenesis and gene silencing. His collaborative work with Dr. Andrew Fire led to the discovery of RNA interference (RNAi), for which they shared the 2006 Nobel Prize in Physiology or Medicine. Together they showed that when C. elegans is exposed to double-stranded ribonucleic acid – dsRNA, a molecule that mimics a signature of viral infection, the worm mounts a sequence-specific silencing reaction that interferes with the expression of cognate cellular RNAs. For the layperson, RNAi is the cell’s search engine; the Google of the cell. Using readily produced short synthetic dsRNAs, researchers can now submit their own RNAi search queries to silence any gene in organisms as diverse as corn and humans. RNAi allows researchers to rapidly “knock out” the expression of specific genes and to thus define the biological functions of those genes. RNAi also provides a potential therapeutic avenue to silence genes that contribute to disease.

Before the Nobel Prize, Dr. Mello’s work on RNAi was recognized with several awards including the National Academy of Sciences Molecular Biology Award, the Canadian Gairdner International Award, the Paul Ehrlich-and Ludwig Darmstaedter Award, and the Dr. Paul Janssen Award for Biomedical Research. He is a member of the National Academy of Sciences, the American Academy of Arts and Sciences, and the American Philosophical Society.

汪阳明(Yangming Wang)博士

北京大学分子医学研究所教授,干细胞与非编码核酸研究室主任

 

学术背景

Dr. Yangming Wang was born in China in 1979. He received his B.S. degree from the Department of Biotechnology at Peking University. He then joined Dr. Scott K. Silverman’s laboratory to study nucleic acid biochemistry at the University of Illinois at Urbana-Champaign, where he obtained his Ph.D. degree in Biochemistry in 2006. After that, he became a postdoctoral fellow studying microRNAs and embryonic stem cells in Dr. Robert H. Blelloch’s laboratory at the Unviersity of California, San Francisco. He became a principal investigator at the Institute of Molecular Medicine, Peking University in 2011. He defined the global function of miRNAs in embryonic stem cells (ESCs) using Dgcr8 knockout ESC model, discovered novel class of small RNAs in ESCs, and identified miR-294/302 family of miRNAs as important regulators of cell cycle and self-renewal in ESCs.

翁志萍(Zhiping Weng)博士

美国麻省大学教授、生物信息学中心主任

国家千人计划重点学科讲座教授

 

学术背景

Zhiping Weng earned a B.S. in Electrical Engineering from University of Science and Technology of China in 1992 and a Ph.D. in Biomedical Engineering from Boston University in 1997. She was an instructor in Biomedical Engineering at Boston University for the next two years, when she had primary responsibility for the development of the Bioinformatics program and the core curriculum in Bioinformatics, until the department recruited her as a tenure-track assistant professor. In 2003, Dr. Weng was promoted to Associate Professor with tenure. Until 2007, Dr. Weng’s research had been focused on developing computational methods for obtaining a predictive understanding of transcriptional regulation and protein-protein interaction. Dr. Weng was recruited to University of Massachusetts Medical School in 2008 to build and direct a new Program in Bioinformatics and Integrative Biology. She is a full professor, with tenure in Department of Biochemistry and Molecular Pharmacology. She continues research on computational analysis of transcriptional regulation and protein-protein interaction. She expands her research into two areas: epigenomics and nucleosome positioning, which play important roles in transcriptional regulation, and function and regulation of small silencing RNAs in metazoan. For more information, please visit Dr. Weng’s lab Website .

杨力(Li Yang)博士

中国科学院上海生命科学研究院计算生物学研究所研究员

 

学术背景

Dr. Li Yang received his Ph.D. degree from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences in 2004. Fascinated by the complexity of RNA regulation, Dr. Yang joined Dr. Sidney Altman’s laboratory at Yale University to study the function of Ribonuclease P (RNase P). Later in Dr. Brenton Graveley’s laboratory at University of Connecticut Health Center, his study has moved on to the high throughput sequencing and bioinformatics to study the transcriptome complexity of the model organism, Drosophila melanogaster, which has allowed him to uncover the global impact of individual RNA binding protein on alternative splicing at single-nucleotide resolution, to develop a comprehensive blueprint of the entire Drosophila transcriptome within modENCODE Consortium, and to apply new methods to explore the repertoire of non-polyadenylated RNAs in human cells. In 2011, Dr. Yang joined CAS-MPG Partner Institute for Computational Biology, Chinese Academy of Sciences to set up his own laboratory, where he and collaborators have successfully identified series of intron-derived non-polyadenylated lncRNAs, either processed and stablized by capping with snoRNAs at both ends, or stablized by forming circular structures. Dr. Yang and collaborators continue using the start-of-the-art deep sequencing technology and computational approaches to pursue his longstanding interest in understanding the complex transcriptome network, including but not limited to alternative splicing, RNA editing and noncoding RNAs .

张晓华(Douglas Zhang)博士

默克研究院生物统计与研究决策科学部(BARDS)主任、亚太区早期发育统计研究主管

中山大学-默沙东(MSD)生物统计与生物信息学联合实验室主任

 

学术背景

Dr. Zhang is the head of Early Development Statistics – Asian Pacific in Merck Research Laboratories. He got his Ph.D. in statistics from Carnegie-Mellon University, PA, USA. He has worked on data analysis for genome-wide RNAi research and microarrays in drug discovery and development for various diseases for many years. He and his colleagues have continuously developed and published novel analytic methods and experimental designs for quality control and hit selection in genome-scale RNAi research in peer-reviewed journals such as Bioinformatics, Nucleic Acids Research, Cell Host&Microbe, et al. His book titled “Optimal High-Throughput Screening: Practical Experimental Design and Data Analysis for Genome-scale RNAi Research” was published by Cambridge University Press, Cambridge, UK in 2011.One of his first-authored articles has been listed among the top 50 most-cited articles inBioinformatics since 2013. Three of his articles (two first-authored and one second-authored) have been listed among the top 50 most-cited articles in Journal of Biomolecular Screening since 2008. One of his co-authored articles had been listed by Nature Medicine in their year-end review on Notable advances in 2008. He has membership in professional competition committee, serves in the Editorial Board in multiple peer-reviewed journals, has served as a referee in many peer-reviewed journals, and has been invited to be an expert evaluator for grant proposals in both the European Union 7th Framework Programme and USA NIH Study Section. He has also been invited to give presentations in many international conferences and seminars in many research universities in both USA and China.He is a committee member for Functional Genomics and System Biology, Chinese Society for Cell Biology.

张戈(Ge Zhang)博士

香港浸会大学骨与关节疾病转化医学研究所副所长

 

学术背景

Previous academic positions held:

• 1995-2000: Resident, Institute of Orthopaedics & Traumatology, Shanghai     University of Chinese Medicine

• 2000-2003: Physician-in-Charge, Institute of Orthopaedics & Traumatology, Shanghai University of Chinese Medicine

• 2003-2007 Postdoctoral Research Fellow, Department of Orthopaedics & Traumatology, The Chinese University of Hong Kong

• 2007-2012 Research Assistant Professor, Department of Orthopaedics & Traumatology, The Chinese University of Hong Kong

 

Previous relevant research work:

• Technical expertise: Bone bioimaging, Bone biology, Bone biomechanics, Bone histomorphometry

• Research area: RNAi-based or phytotherapy-based translational research in osteoporosis, osteonecrosis, facture repair and arthritis

张弓(Gong Zhang)博士

暨南大学生命与健康工程研究院教授

 

学术背景

Dr. Gong Zhang has been working on Translatomics, i.e. rules and biological implications of translational control, especially in the global level. He proposed that the amino acid sequence is not sufficient for protein folding, and translational pausing is the additional and essential information for co-translational folding. This renewed the Anfinsen’s Dogma. He revealed, for the first time, the quantitative rule of transmission from mRNA to protein, and thus solved the open question for 55 years since Central Dogma was proposed. He also developed the extremely accurate and robust read mapping algorithm for next-generation sequencing.

朱景德(Jingde Zhu)博士

安徽肿瘤医院教授,上海市肿瘤研究所研究员

 

学术背景

Resarch Interests and acadmic achievements:

The research in Jingde Zhu’s group is focused at both basci and translational aspects of cancer biology viewed from DNA methylation perspective. They are supported by both Central and local governments in China and the 6th Frame program from Europe. Dr. Zhu has published over fifty paper in the peer-review journals, including those in Cell, Nature, Nature Biotechnology, Cell Research, Cancer Research, NAR, CCR, Oncogene, JBC, Cancer Gene Therapy, , and etc. He is the inventor of one approved US patent and two filed US PCT. He is also the inventor of four approved and two filed patents in China. He has organized four international conferences and spoken in over 40 onferences in life sciences in last five years.

 

A Brief Education and Research Experiences:

1970-1973 University education in Biology from Anhui Normal University, China
1973-1978 Lecturer in Medical Microbiology, Benpu Medical College, China
1978-1980 Master Degree education in Academia Sinica
1980-1985 Ph.D education in Beatson Institute for Cancer Research, Glasgow University, UK
1985-1990 P.I, Associated Professor, Shanghai Inst of Cell Biology, Academia Sinica
1990-June,2001   Senior scientist, P.I, in three academic and one Biotech firm in UK

       论坛起源于广州核酸国际论坛,在政府及各界支持下已经连续举办了五届,历届大会报告人汇集诺贝尔奖得主在内的全球顶级科学家、学者和企业家,论坛聚焦精准医学、生命科学、基因治疗、核酸药开发等最新热点进展,分享来自全球范围内核酸研究及应用的重大成果和进展。中国核酸国际论坛(CNAF)的知名度与影响力也受到国际学术界与企业界的高度赞誉,被誉为中国核酸研究与产业化的重要风向标。